Western Blot:Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
Article Snippet: .. Protein Primary antibody Dilution Source ACTB/β-actin Mouse monoclonal (8H10D10C4) to ACTB/β-actin 1:1000 Cell Signaling, #3700S ATG5 Rabbit polyclonal to ATG5 1:1000 Cell Signaling, #2630S BNIP3L Rabbit monoclonal (D4R4B) to BNIP3L 1:1000 Cell signaling, #12396 Flag Rabbit monoclonal (D6W5B) to DYKDDDDK Mouse monoclonal (9A3) to DYKDDDDK 1:1000 Cell signaling, #14793 Cell signaling, #8146 HA Rabbit monoclonal (C29F4) to HA-Tag 1:1000 Cell signaling, #3724 His Mouse monoclonal (4D11) to His tag 1:1000 for WB, 1:200 for microscopy Abcam, #ab5000 HIV gp120 Goat polyclonal to HIV-1 gp120 1:1000 Abcam, #ab21179 HIV Nef Mouse monoclonal to HIV-1 Nef 1:1000 ThermoFisher Sci, #MA1–71505 HIV p24 Mouse monoclonal (183-H12-5C) to HIV-1 p24 1:1000 NIH-ARP, #ARP-3537 HIV Vif Mouse monoclonal (319) to HIV-1 Vif 1:1000 Abcam, #ab66643 HIV Vpr Rabbit polyclonal to HIV-1 Vpr 1:1000 ThermoFisher Sci, #51143–1-AP HIV Vpu Rabbit polyclonal to HIV-1 Vpu 1:1000 NIH-ARP, #ARP-969 LC3B Rabbit polyclonal to LC3B 1:1000 Cell Signaling, #2775S mCherry Rabbit monoclonal (E5D8F) to mCherry 1:1000 Cell Signaling, #43590 Mouse IgG Goat anti-mouse (H + L) secondary HRP 1:2000 ThermoFisher Sci, #31430 Mouse IgG1 Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-21124 Mouse IgG1 Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-21121 Mouse IgG2a Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-21131 Mouse IgG2a Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-21134 Myc Mouse monoclonal (9B11) to Myc tag 1:1000 for WB, 1:100 for microscopy Cell signaling, #2276 NBR1 Rabbit monoclonal (E6Q3F) to NBR1 1:1000 Cell signaling, #20145S NDP52 Rabbit polyclonal to NDP52 1:1000 for WB, 1:100 for microscopy Abcam, #ab68588 Optineurin (OPNT) Rabbit monoclonal (E4P8C) to Optineurin 1:1000 Cell signaling, #70928 Rabbit IgG Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-11008 Rabbit IgG Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-11011 SQSTM1/p62 Mouse monoclonal (2C11) to SQSTM1/p62 Rabbit monoclonal (D1Q5S) to SQSTM1/p62 1:1000 for WB, 1:100 for microscopy Abcam, #ab56416 Cell signaling, #39749 TAX1 BP1 Rabbit monoclonal (D1D5) to TAX1 BP1 1:1000 for WB, 1:100 for microscopy Cell signaling, #5105 Ubiquitin Rabbit monoclonal (4B10B2) to Ubiquitin 1:1000 Cell signaling, #43124S https://doi.org/10.1371/journal.ppat.1014020.t002 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014020 March 2, 2026 16 / 35 Fig 8. ..
Microscopy:Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
Article Snippet: .. Protein Primary antibody Dilution Source ACTB/β-actin Mouse monoclonal (8H10D10C4) to ACTB/β-actin 1:1000 Cell Signaling, #3700S ATG5 Rabbit polyclonal to ATG5 1:1000 Cell Signaling, #2630S BNIP3L Rabbit monoclonal (D4R4B) to BNIP3L 1:1000 Cell signaling, #12396 Flag Rabbit monoclonal (D6W5B) to DYKDDDDK Mouse monoclonal (9A3) to DYKDDDDK 1:1000 Cell signaling, #14793 Cell signaling, #8146 HA Rabbit monoclonal (C29F4) to HA-Tag 1:1000 Cell signaling, #3724 His Mouse monoclonal (4D11) to His tag 1:1000 for WB, 1:200 for microscopy Abcam, #ab5000 HIV gp120 Goat polyclonal to HIV-1 gp120 1:1000 Abcam, #ab21179 HIV Nef Mouse monoclonal to HIV-1 Nef 1:1000 ThermoFisher Sci, #MA1–71505 HIV p24 Mouse monoclonal (183-H12-5C) to HIV-1 p24 1:1000 NIH-ARP, #ARP-3537 HIV Vif Mouse monoclonal (319) to HIV-1 Vif 1:1000 Abcam, #ab66643 HIV Vpr Rabbit polyclonal to HIV-1 Vpr 1:1000 ThermoFisher Sci, #51143–1-AP HIV Vpu Rabbit polyclonal to HIV-1 Vpu 1:1000 NIH-ARP, #ARP-969 LC3B Rabbit polyclonal to LC3B 1:1000 Cell Signaling, #2775S mCherry Rabbit monoclonal (E5D8F) to mCherry 1:1000 Cell Signaling, #43590 Mouse IgG Goat anti-mouse (H + L) secondary HRP 1:2000 ThermoFisher Sci, #31430 Mouse IgG1 Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-21124 Mouse IgG1 Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-21121 Mouse IgG2a Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-21131 Mouse IgG2a Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-21134 Myc Mouse monoclonal (9B11) to Myc tag 1:1000 for WB, 1:100 for microscopy Cell signaling, #2276 NBR1 Rabbit monoclonal (E6Q3F) to NBR1 1:1000 Cell signaling, #20145S NDP52 Rabbit polyclonal to NDP52 1:1000 for WB, 1:100 for microscopy Abcam, #ab68588 Optineurin (OPNT) Rabbit monoclonal (E4P8C) to Optineurin 1:1000 Cell signaling, #70928 Rabbit IgG Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-11008 Rabbit IgG Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-11011 SQSTM1/p62 Mouse monoclonal (2C11) to SQSTM1/p62 Rabbit monoclonal (D1Q5S) to SQSTM1/p62 1:1000 for WB, 1:100 for microscopy Abcam, #ab56416 Cell signaling, #39749 TAX1 BP1 Rabbit monoclonal (D1D5) to TAX1 BP1 1:1000 for WB, 1:100 for microscopy Cell signaling, #5105 Ubiquitin Rabbit monoclonal (4B10B2) to Ubiquitin 1:1000 Cell signaling, #43124S https://doi.org/10.1371/journal.ppat.1014020.t002 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014020 March 2, 2026 16 / 35 Fig 8. ..
Ubiquitin Proteomics:Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
Article Snippet: .. Protein Primary antibody Dilution Source ACTB/β-actin Mouse monoclonal (8H10D10C4) to ACTB/β-actin 1:1000 Cell Signaling, #3700S ATG5 Rabbit polyclonal to ATG5 1:1000 Cell Signaling, #2630S BNIP3L Rabbit monoclonal (D4R4B) to BNIP3L 1:1000 Cell signaling, #12396 Flag Rabbit monoclonal (D6W5B) to DYKDDDDK Mouse monoclonal (9A3) to DYKDDDDK 1:1000 Cell signaling, #14793 Cell signaling, #8146 HA Rabbit monoclonal (C29F4) to HA-Tag 1:1000 Cell signaling, #3724 His Mouse monoclonal (4D11) to His tag 1:1000 for WB, 1:200 for microscopy Abcam, #ab5000 HIV gp120 Goat polyclonal to HIV-1 gp120 1:1000 Abcam, #ab21179 HIV Nef Mouse monoclonal to HIV-1 Nef 1:1000 ThermoFisher Sci, #MA1–71505 HIV p24 Mouse monoclonal (183-H12-5C) to HIV-1 p24 1:1000 NIH-ARP, #ARP-3537 HIV Vif Mouse monoclonal (319) to HIV-1 Vif 1:1000 Abcam, #ab66643 HIV Vpr Rabbit polyclonal to HIV-1 Vpr 1:1000 ThermoFisher Sci, #51143–1-AP HIV Vpu Rabbit polyclonal to HIV-1 Vpu 1:1000 NIH-ARP, #ARP-969 LC3B Rabbit polyclonal to LC3B 1:1000 Cell Signaling, #2775S mCherry Rabbit monoclonal (E5D8F) to mCherry 1:1000 Cell Signaling, #43590 Mouse IgG Goat anti-mouse (H + L) secondary HRP 1:2000 ThermoFisher Sci, #31430 Mouse IgG1 Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-21124 Mouse IgG1 Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-21121 Mouse IgG2a Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-21131 Mouse IgG2a Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-21134 Myc Mouse monoclonal (9B11) to Myc tag 1:1000 for WB, 1:100 for microscopy Cell signaling, #2276 NBR1 Rabbit monoclonal (E6Q3F) to NBR1 1:1000 Cell signaling, #20145S NDP52 Rabbit polyclonal to NDP52 1:1000 for WB, 1:100 for microscopy Abcam, #ab68588 Optineurin (OPNT) Rabbit monoclonal (E4P8C) to Optineurin 1:1000 Cell signaling, #70928 Rabbit IgG Goat polyclonal (Alexa-488 conjugated) 1:500 ThermoFisher Sci, #A-11008 Rabbit IgG Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Sci, #A-11011 SQSTM1/p62 Mouse monoclonal (2C11) to SQSTM1/p62 Rabbit monoclonal (D1Q5S) to SQSTM1/p62 1:1000 for WB, 1:100 for microscopy Abcam, #ab56416 Cell signaling, #39749 TAX1 BP1 Rabbit monoclonal (D1D5) to TAX1 BP1 1:1000 for WB, 1:100 for microscopy Cell signaling, #5105 Ubiquitin Rabbit monoclonal (4B10B2) to Ubiquitin 1:1000 Cell signaling, #43124S https://doi.org/10.1371/journal.ppat.1014020.t002 PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1014020 March 2, 2026 16 / 35 Fig 8. ..
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